|a英文題名:Lignocellulolytic Enzyme Activities of Xylaria sp. PU02 by Solid, Semi-solid and Liquid State Cultivation
|a炭角菌為一種子囊真菌，通常生長於樹木、樹皮或腐木上，大多數為腐生菌，具有木質纖維分解酵素之活性。另外，由於炭角菌種類多樣，也發現其代謝產物具抗氧化、抗腫瘤生長與抑菌等功能。在本研究中，自靜宜大學校園腐木上採集炭角菌子實體，分離出炭角菌PU02，分別以固態、半固態與液態方式培養，探討不同培養條件下木質素降解酵素與纖維素分解酵素之活性，包括漆氧化酶（laccase）、錳過氧化酶（manganese peroxidase）、木質素過氧化酶（lignin peroxidase）、內切型纖維素酶（carboxymethyl cellulase）、β-葡萄糖苷酶（β-glucosidase）與聚木醣酶（xylanase）。實驗上使用麥麩與黃豆殼以1:1比例混合作為培養基質，利用旋轉式發酵設備進行固態與半固態培養，在操作上同時採用靜置/旋轉與通氣/不通氣等培養條件。另外，利用搖瓶震盪培養方式進行液態培養。結果顯示，在固態培養中，laccase、manganese peroxidase與β-glucosidase酵素活性最高，分別達到0.64、0.14與0.2 U/g substrate，而carboxymethyl cellulase與xylanase酵素活性可達到16.5與20.1 U/g substrate。在半固態培養中，產生較高lignin peroxidase與carboxymethyl cellulase酵素活性，可達到0.61與16.0 U/g substrate，其他酵素活性皆很低。在液態培養時，carboxymethyl cellulase與xylanase酵素活性較高，達到18.0與38.7 U/g substrate。因此，不同培養方式會影響炭角菌產生不同之酵素活性，在固態培養時炭角菌PU02可產生較多樣化之酵素。未來可利用炭角菌PU02生產多樣化酵素，應用於農業廢棄物處理、環境污染處理、生質能源生產等用途上。|uXylaria species, the wood-colonizing ascomycetes, caused wood decay due to their lignin-degrading enzymes and cellulose hydrolysis enzymes. They usually grow on trees, bark or rotten wood. Species diversity of Xylaria also produce diverse metabolites with antioxidant, antimicrobial and antitumor activities. Xylaria sp. PU02 was a newly isolated strain from decayed wood in Providence University campus. In this study, the activities of lignocellulolytic enzymes of PU02 by solid-state, semi-solid state and liquid state cultivation were investigated, including laccase, manganese peroxidase, lignin peroxidase, carboxymethyl cellulase, β-glucosidase and xylanase. A mixture of wheat bran and soybean hull at a ratio of 1:1 was used as substrate in all cultivations. In solid-state and semi-solid state cultivations, rotary fermenter was used with the combination of static/ rotating and aeration/ non-aeration operations. Liquid state cultivations were carried out in shake flasks. The results showed that PU02 produced more diverse enzymes by solid state cultivation with the activities of laccase, manganese peroxidase, β-glucosidase, carboxymethyl cellulase and xylanase, which were 0.64, 0.14, 0.2, 16.5 and 20.1 U/g substrate, respectively. The activities of laccase, manganese peroxidase and β-glucosidase were the highest in comparison with those of the other types of cultivation. In semi-solid state cultivation, the activities of lignin peroxidase and carboxymethyl cellulase were 0.61 and 16.0 U/g substrate, but other enzymes activities were very low. In liquid state cultivation, the highest activities of carboxymethyl cellulose and xylanase were obtained that reached 18.0 and 38.7 U/g substrate. Hence, PU02 produced diverse and different kinds of lignocellulolytic enzymes under different types of cultivation, especially in the solid state cultivation. In the future, lignocellulolytic enzymes from PU02 could be applied in agricultural waste treatment, environmental pollution treatment and bioenergy production.
|aLignocellulolytic Enzyme Activities of Xylaria sp. PU02 by Solid, Semi-solid and Liquid State Cultivation