|a英文題名:Studies on the Antioxidant and Anti-leukemia Activities of Essential Oils from Zanthoxylum ailanthoides Sieb & Zucc
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|a指導教授:周淑姿
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|a含參考書目
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|a碩士論文--靜宜大學食品營養學系
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|a食茱萸 (Zanthoxylum ailanthoides Sieb & Zucc.) 為芸香科花椒屬植物，是傳統的中草藥之ㄧ，俗稱紅刺蔥、食茱萸等。研究指出食茱萸萃取物具有良好抗氧化活性及抗腫瘤之功效，故本研究目的為探討經蒸餾法 (steam distillation) 萃取的食茱萸精油 (Zanthoxylum ailanthoides Sieb & Zucc. essential oil, ZA-EO ) 其化學組成、抗氧化及抗癌活性。利用氣相層析質譜儀 (gas chromatography mass spectrometry, GC-MS) 分析精油之主要成分，結果顯示精油成份含有高量的含氧萜烯類化合物，尤以沉香醇 (β-linalool) 含量高達 40.95 ％，桉油醇 (1,8-cineole) 次之，約為 16.17 ％。首先以體外試驗測定ZA-EO及其組成分之抗氧化能力，結果顯示ZA-EO、β-linalool及1,8-cineole皆具有良好的螯合亞鐵與抑制脂質過氧化生成之能力。在細胞實驗方面，利用MTT試驗評估ZA-EO及組成分之化合物抗癌活性，結果顯示ZA-EO、β-linalool及1,8-cineole對小鼠血癌細胞 (WEHI-3) 生長具有良好抑制效果。並利用DAPI螢光染色法及彗星試驗來評估WEHI-3細胞損傷，結果顯示ZA-EO、β-linalool及1,8-cineole之添加會造成WEHI-3細胞DNA受損而走向凋亡。流式細胞儀結果得知ZA-EO (7.5 μg/ml) 、β-linalool (100 μg/ml) 及1,8-cienole (100 μg/ml) 添加下，會增加細胞內ROS含量及鈣離子的釋放，使細胞走向凋亡。此外，ZA-EO、β-linalool及1,8-cineole之添加亦會改變細胞內氧化-抗氧化之恆定，降低glutathione 含量及 catalase (CAT) 活性，並增加glutathione peroxidase (GPx) 及malondialdehyde (MDA) 之生成，但對superoxide dismutase (SOD) 活性並無影響。綜合上述結果得知ZA-EO、β-linalool及1,8-cineole在體外皆具有抗氧化力，且能有效抑制WEHI-3細胞之生長並能造成受測細胞DNA之損傷，而ZA-EO、β-linalool及1,8-cineole也能改變WEHI-3細胞氧化-抗氧化系統之恆定並誘導WEHI-3細胞走向凋亡，因此可以推測β-linalool及1,8-cineole是食茱萸精油之抗氧化及抗癌活性主要的提供者。|uThe leaf essential oil of Zanthoxylum ailanthoides Sieb & Zucc., a traditional Chinese herbal medicine that is belonging to the Rutaceae family was extracted by hydrodistillation. The composition of the volatile fraction of this essential oil was determined by GC–MS analyses. We have identified 17 compounds, of which β-linalool (40.95%), 1,8-cineole (16.17%) are the main components. In this study, we examined the biological activities including antioxidant and anti-tumour of Zanthoxylum ailanthoides Sieb & Zucc. essential oil (ZA-EO) , β-linalool and 1,8-cineole. The results demonstrated that ZA-EO, β-linalool and 1,8-cineole possessed antioxidative characteristics including Fe2+-chelating ability and lipid peroxidation inhibitory activities. In cell model, MTT assay was carried out to determine on the cancer toxicity of ZA-EO, β-linalool and 1,8-cineole. The results indicated that ZA-EO, β-linalool and 1,8-cineole, had inhibitory effects on cell viability of mouse BALB/c myelomonocytic leukemia (WEHI-3) cells. Furthermore, ZA-EO, β-linalool and 1,8-cineole induced DNA damage and apoptosis confirmed by DAPI staining and comet assay in WEHI-3 cells. Flow cytometry assay demonstrated that ZA-EO, β-linalool and 1,8-cineole stimulated cellular reactive oxygen species (ROS) production and calcium releasing. Moreover, ZA-EO, β-linalool and 1,8-cineole increased malondialdehyde levels and glutathione peroxidase (GPx) activity and decreased glutathione levels and catalase (CAT) activity in WEHI-3 cells. In conclusion, our results revealed that the ZA-EO, β-linalool and 1,8-cineole possessed antioxidative activity, inhibited cell proliferation and induced DNA damage in WEHI-3 cells. In addition, ZA-EO, β-linalool and 1,8-cineole induced unbalance of antioxidative defense systems then toward to apoptosis in WEHI-3 cells. Hence, there results suggested that the ZA-EO has a potent inhibitory effect against leukemia cell growth and β-linalool and 1,8-cineole may be responsible for the functional ingredients in ZA-EO.
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|aStudies on the Antioxidant and Anti-leukemia Activities of Essential Oils from Zanthoxylum ailanthoides Sieb & Zucc
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