|a英文題名:Antioxidant and Neuroprotection Activities of Myrciaria Cauliflora Seed Extracts
|a活性氧化物質的產生和生物體抗氧化系統之間的不平衡會造成氧化壓力並導致神經退化性疾病，本研究目的為探討嘉寶果果籽萃取物之抗氧化能力及對神經細胞之保護功效。嘉寶果果籽分別以95 %乙醇、50 %乙醇和熱水進行萃取，經減壓濃縮和冷凍乾燥製成粉末。抗氧化成分分析包括總多酚、總花青素、總類黃酮和白藜蘆醇之含量；而抗氧化活性主要分析DPPH(1,1-diphenyl-2-picrylhydrazyl)自由基清除能力、還原力和螯合二價鐵離子能力。此外以Neuro-2a細胞作為神經細胞模式，以H2O2誘導細胞氧化性損傷後，以MTT分析細胞存活率、使用H2DCF-DA染劑測定活性氧化物質(reactive oxygen species, ROS)生成量，最後分析細胞抗氧化酵素活性及抗氧化物質含量。結果顯示50%乙醇萃取物含有最高量之總多酚(164.19 ± 4.46 mg gallic acid equivalent/g)、總類黃酮(17.48 ± 0.01 mg rutin equivalent/g)和總花青素(1.87± 0.04 mg/g),及最佳之抗氧化能力。DPPH自由基清除能力、還原力和螯合二價亞鐵離子之EC50分別為0.20 ± 0.00 mg/ml、0.13 ± 0.00 mg/ml和0.09 ± 0.00 mg/ml。細胞實驗顯示，95 %乙醇萃取物可提升24% 存活率並且可降低ROS生成量和促進catalase之活性。綜合上述研究得知，嘉寶果果籽萃取物含豐富多酚類化合物故有良好之抗氧化活性，可降低ROS生成量，減少氧化壓力，以保護N2a神經細胞。|uAn imbalance between production of reactive oxygen species and antioxidant system can cause oxidative stress and lead to neurodegenerative diseases. The objective of this study was to investigate antioxidant activity and neuroprotection effects of Myrciaria cauliflora (Jaboticaba) seeds extracts in vitro. Jaboticaba seeds were extracted with 95% ethanol, 50% ethanol and hot water, respectively. The extracts were vacuum-concentrated followed by lyophilizated. Antioxidant components in extracted powder, such as total polyphenol, total anthocyanins, total flavonoids, and resveratrol, were analyzed. Reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and ferrous ion chelation ability were determined to evaluate the antioxidant capacity of extracts. To measure neuroprotection effect, oxidative stress was induced by H2O2 in N2a mouse neuroblastoma cells. The cell viability was analyzed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, while the amount of ROS production was assayed with H2DCF-DA stain, and the antioxidant status including the activities of cellular antioxidant enzymes and antioxidant contents were determined as well. The results showed that 50% ethanol extract contained the highest amount of total polyphenols (164.19 ± 4.46 mg gallic acid equivalent/g), total flavonoids (17.48 ± 0.01 mg rutin equivalent/g) and total anthocyanins (1.87 ± 0.04 mg/g). The EC50 of antioxidant properties of 50% ethanol extract expressed as DPPH radical scavenging ability, reducing power (reach OD 0.5 at 700nm) and ferrous ion chelation were 0.20 ± 0.00 mg/ml, 0.13 ± 0.00 mg/ml and 0.09 ± 0.00 mg/ml, respectively. Compare to untreated N2a cell line, N2a treated with 95% ethanol extract showed higher viability, lower ROS production and greater catalase activity. In conclusion, Jaboticaba seed extracts was proved to have neuroprotection effect by its antioxidant activity.
|aAntioxidant and Neuroprotection Activities of Myrciaria Cauliflora Seed Extracts